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Rapid Detection of Human Respiratory Syncytial Virus A and B by Duplex Real-Time RT-PCR

Respiratory syncytial virus (RSV) is a typical reason for acute respiratory illness worldwide, particularly in younger youngsters. The World Well being Group (WHO) has initiated an RSV Surveillance Pilot program that goals to carry out worldwide RSV surveillance, requiring the event of dependable and speedy molecular strategies to detect and determine RSV. A duplex real-time …
Human Saliva as a Diagnostic Specimen for Early Detection of Inflammatory Biomarkers by Real-Time RT-PCR

These days human saliva is extra incessantly studied as a non-invasive, stress-free, and preferable diagnostic materials than blood. Supporting evidences acknowledge saliva as a mirror that displays the physique’s bodily state. Quite a few research have additionally demonstrated the presence and use of RNA derived from saliva within the early prognosis of illness by real-time …
Validation of a Novel Diagnostic Approach Combining the VersaTREK™ System for Recovery and Real-Time PCR for the Identification of Mycobacterium chimaera in Water Samples

Mycobacterium chimaera is an rising pathogen related to endocarditis and vasculitis following cardiac surgical procedure. Though it will probably take as much as 6-Eight weeks to tradition on selective strong media, culture-based detection stays the gold customary for analysis, so extra fast strategies are urgently wanted. For the current research, we processed environmental M. chimaera contaminated simulates at …
Validation of a novel FRET real-time PCR assay for simultaneous quantitative detection and discrimination of human Plasmodium parasites

Rising numbers of vacationers coming back from endemic areas, migrants, and refugees have led to a big rise within the variety of imported malaria circumstances in non-endemic nations. Actual- time PCR serves as a wonderful diagnostic device, particularly in areas the place expertise in microscopy is restricted. A novel fluorescence resonance power transfer-based real-time PCR …
Focus Simplexa Controls
The DiaSorin Molecular Simplexa™ COVID-19 Direct real-time RT-PCR assay is intended for use on the LIAISON® MDX instrument for the in vitro qualitative detection of nucleic acid from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in nasopharyngeal swabs (NPS), nasal swabs (NS), nasal wash/aspirate (NW) or bronchoalveolar lavage (BAL) specimens from individuals suspected of COVID-19 …
Rapid differential detection of subtype H1 and H3 swine influenza viruses using a TaqMan-MGB-based duplex one-step real-time RT-PCR assay

Swine influenza is an economically vital respiratory illness in swine, nevertheless it additionally consistently poses a risk to human well being. Due to this fact, creating fast, delicate, and environment friendly detection strategies for swine influenza virus (SIV) is vital. By aligning the haemagglutinin (HA) gene sequences of SIVs circulating in China over a 10-year …
Critical parameters of real time reverse transcription polymerase chain reaction (RT-PCR) diagnostics: sensitivity and specificity for bluetongue virus

A brand new variant of bluetongue virus serotype 3, BTV3 ITL 2018 (right here named: BTV3), was included in serial dilutions within the BT Proficiency Take a look at 2020. Though the OIE-recommended panBTV actual time RT-PCR take a look at focusing on genome section 10 (Seg-10) detected this variant, we confirmed that reverse transcription …
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Recent Posts
- Compare rec. lab reagents for research
- Rapid Detection of Human Respiratory Syncytial Virus A and B by Duplex Real-Time RT-PCR
- Human Saliva as a Diagnostic Specimen for Early Detection of Inflammatory Biomarkers by Real-Time RT-PCR
- Validation of a Novel Diagnostic Approach Combining the VersaTREK™ System for Recovery and Real-Time PCR for the Identification of Mycobacterium chimaera in Water Samples
- Validation of a novel FRET real-time PCR assay for simultaneous quantitative detection and discrimination of human Plasmodium parasites