Rapid differential detection of subtype H1 and H3 swine influenza viruses using a TaqMan-MGB-based duplex one-step real-time RT-PCR assay

Rapid differential detection of subtype H1 and H3 swine influenza viruses using a TaqMan-MGB-based duplex one-step real-time RT-PCR assay
June 26, 2021 Benjamin 0 Comments

Swine influenza is an economically vital respiratory illness in swine, nevertheless it additionally consistently poses a risk to human well being. Due to this fact, creating fast, delicate, and environment friendly detection strategies for swine influenza virus (SIV) is vital. By aligning the haemagglutinin (HA) gene sequences of SIVs circulating in China over a 10-year …

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Critical parameters of real time reverse transcription polymerase chain reaction (RT-PCR) diagnostics: sensitivity and specificity for bluetongue virus

Critical parameters of real time reverse transcription polymerase chain reaction (RT-PCR) diagnostics: sensitivity and specificity for bluetongue virus
June 26, 2021 Benjamin 0 Comments

A brand new variant of bluetongue virus serotype 3, BTV3 ITL 2018 (right here named: BTV3), was included in serial dilutions within the BT Proficiency Take a look at 2020. Though the OIE-recommended panBTV actual time RT-PCR take a look at focusing on genome section 10 (Seg-10) detected this variant, we confirmed that reverse transcription …

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